The Victorian Government said it was part of its plans to make public transport easier and more affordable.
Tap and go will be switched on in stages across Victoria’s rail network.
On June 7, it went live on the Sunbury, Pakenham, Cranbourne, Metro Tunnel, Werribee, Williamstown, Sandringham, Frankston, Stony Point, Bendigo, Gippsland and Geelong lines.
On June 14, it was expanded to the Mernda, Hurstbridge, Lilydale, Belgrave, Alamein and Glen Waverley lines.
The ability to tap and go on the Seymour line will also continue.
Following this rollout, passengers will be able to tap on and travel using a bank card, smartphone or smartwatch at every myki-enabled train station across Victoria.
More than 88,500 tap and go trips have been taken since trials began in March, with four in five of those made using a phone or smartwatch.
Station staff will be on hand to help passengers as the rollout continues.
Victorian Public and Active Transport Minister Gabrielle Williams said there would be more to say in the coming weeks about the next stage of the rollout to trams and buses.
"Victorians are already saving money with half-price fares. Now we’re making public transport easier to use, too,“ she said.
"Tap and go means one less card in your wallet and one less thing to worry about on your journey.“
Concession passengers, seniors and those under-18 should continue using their concession or free Youth myki cards.
Passengers travelling where myki is not yet available should continue using a paper ticket or eTicket.
Passengers who prefer to use their myki can continue to do so.
More information is available at transport.vic.gov.au/tap-and-go/