The rally will be held outside the Russian embassy in Canberra on Saturday to protest Moscow's war against Ukraine after Russian troops began their offensive in February 2022.
They will be joined by independent senator David Pocock and ACT Attorney-General Shane Rattenbury.
Marking the occasion, the coalition called on the Albanese government to reverse its decision to bury Australia's dumped MRH-90 Taipan helicopters and to instead donate them to Ukraine as requested in 2023.
The government maintains there are none in flying condition.
In a joint statement, Opposition Leader Peter Dutton alongside his opposition foreign and defence spokesmen, also urged the reinstatement of an Australian embassy in Kyiv to join the more than 67 other diplomatic missions that have already returned.
"On this second anniversary, we salute the unwavering strength of Ukrainians which has kept the belligerent advances of Russia at bay," they said.
"We mourn with  Ukrainians for the displacement, casualties and fatalities they have suffered at the hands of a murderous dictator."
Senator Pocock said the international community needed to exert maximum pressure, while providing all possible support to Ukraine.
"Their friends and supporters here in Canberra have shown up week in and week out in solidarity, refusing to be silent and keeping attention on Ukraine's plight," he said.
Ukrainian Ambassador Vasyl Myroshnychenko met other European envoys on Friday in a show of solidarity to mark the anniversary.
Mr Myroshnychenko will address the National Press Club in Canberra next week.
The British High Commission has been lit up in Ukraine's colours of blue and yellow as High Commissioner Vicki Treadell met with the Ukrainian ambassador.
It will remain illuminated over the weekend.
14°C