The footage, which runs about eight hours, includes Timberlake's initial stop after Sag Harbor police said he ran a stop sign in the village centre, veered out of his lane and got out of his BMW smelling of alcohol that June.
The NSYNC singer-turned-solo artist and actor tells officers he had consumed one martini and had been following friends home in the former whaling village, which is among the affluent beach towns of the Hamptons, about 160km east of New York City.
When an officer asks why he is in town, Timberlake says, "I'm on a world tour."
"Doing what?" the officer asks.
"Hard to explain," Timberlake says.
After stammering a bit, he says "World tour. I'm Justin Timberlake."
The officer eventually responds: "You are Justin Timberlake? Do you have a licence with you?"
Timberlake, who ultimately pleaded guilty to a lesser charge, is asked by officers to walk heel-to-toe in a straight line on the road and lift one leg. At times he seems flustered listening to the instructions. He apologises to officers and tells them his heart is racing.
"I'm a little nervous," Timberlake says at one point.
In the back seat of the police car, he asks: "Why are you arresting me?"
Back at the police station, Timberlake is informed he will be held overnight, to which he says, "I'm going to be here all night? You guys are wild, man."
He asks the officer to keep the light on in the cell as they lock the door.
The release by Sag Harbor Police comes after the village and Timberlake's lawyers agreed to disclose a redacted version of the footage. The Associated Press was among several media outlets that filed a records request seeking the release of the video.
Timberlake's lawyers had sued to block the release of the video, arguing it would "devastate" Timberlake's privacy by revealing "intimate, highly personal, and sensitive details". They also said it would cause "severe and irreparable harm" to his reputation by subjecting him to "public ridicule and harassment".
But in a joint filing with the village on Friday, Timberlake's lawyers acknowledged the video "does not constitute an unwarranted invasion of personal privacy under" the state's public information law and agreed to its release.
Timberlake's lawyers and representatives didn't immediately respond to emails seeking comment on Friday.
Sag Harbor officials, in a statement provided by the village's lawyer, Vincent Toomey, said they are pleased the matter was resolved and they were able to comply with state public records law.
Timberlake pleaded guilty to impaired driving in September 2024.
The Tennessee native agreed to give a public safety announcement against the perils of drunken driving as part of the plea deal that knocked down his initial misdemeanour charge to a non-criminal traffic violation.
He was also sentenced to a $US500 ($A700) fine, 25 hours of community service and a 90-day suspension of his licence.
