Victorian state president Warren Pickering, federal party leader Pauline Hanson and more than 750 supporters gathered at Melbourne's Crown conference centre on Saturday.
A number of policies have been announced at the launch, with a promise to boost powers for police atop the list.
Inside the event, there has been no shortage of merchandise on display including T-shirts and caps emblazoned with the slogan "MAGA Make Albo Go Away".
Most of the party's candidates are also in attendance.
Activists declared their intention to protest outside in the lead-up to Saturday, while a major rally is also planned for later in October.
Announcing the agenda for the launch on Friday, Mr Pickering told radio 3AW Melbourne 95 per cent of candidates and a full policy platform would be presented.
One in three Victorians rate crime as a top issue, higher than in any other state, according to the latest Mood of the Nation report released this week by public affairs and strategic communications consultancy SEC Newgate.
The polling found voters nominated the coalition as being best placed to manage crime and community safety, compared with 16 per cent for Labor, with 19 per cent selecting One Nation.
Crime and bail remained a top priority for One Nation, Mr Pickering said on Friday.
"(It) is the key issue in the state right now and people need to feel safe in their homes and places of work once more," he said.
"You make that happen by taking a pragmatic approach, not an expensive one, to ensuring we get more police on the streets, get our empty stations manned and ensure bail laws and sentences are fitting with the crimes."
Protest spokeswoman Julie Monteiro said the demonstrators intended to let One Nation supporters know their "racist party will be challenged wherever it appears".
"Migrants are welcome, racists are not," she said.
Saturday marks the first major test for Mr Pickering, who faced intense scrutiny after admitting to taking speed, ecstasy and marijuana before joining the Australian Defence Force at the age of 28.
During a heated press conference on September 8, the state leader said his past was littered with "mistakes" but insisted he was the right person for the top job.
His admissions came after The Australian newspaper published a series of allegations raised by his former partner.
Mr Pickering, who is standing for the lower house seat of Pakenham at the November state election, previously ran in the 2022 state election and for the federal Senate in 2025 without success.
The latest Resolve Political Monitor, conducted in September and published by Nine Newspapers, shows Labor's primary vote was steady at 25 per cent, the coalition's pushed up one point to 27 per cent and One Nation is still at 23 per cent.
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